WebMar 4, 1994 · Arg-DHFR, a dihydrofolate reductase bearing an amino-terminal (N-terminal) arginine, is long-lived in the yeast Saccharomyces cerevisiae, even though arginine is a destabilizing residue in the N-end rule of protein degradation.
Regulation of the one carbon folate cycle as a shared
WebNov 1, 2012 · Yeast expressing wild-type DJ-1 inserted in DHFR (dhDJ1) grew similar to yeast expressing mutant forms at 30°, while at 37°, dhDJ1 M26I and dhDJ1 L166P showed a slight reduction in growth (∼61–69% of wild-type at 48 hr) . As with SOD-1, addition of a destabilizing P66L mutation in DHFR reduced growth, such that at 30° the disease … We constructed plasmids using restriction-free cloning methods36. For a complete list of plasmids, see Supplementary Table 7. The I-SceI restriction site sequence was agttacgctagggataacagggtaatatag. The protein linker sequences (which also served as the generic recombination sites) were as follows: L1, 5′ … See more Total number of genes annotated in SGD currently stands at 6,075, excluding dubious ones. Because of the structure of our tagging cassette, we did not attempt to tag the 62 yeast proteins that include an N′ intron or the 250 … See more The BY4741 laboratory strain38, which is the basis for most systematic yeast libraries, was used as the master strain for the collection. The … See more Donor strains were constructed on the background of an SGA41 compatible query strain and contained a galactose-induced I-SceI … See more To select pristine strains to be included in the final library, we picked four clones for each gene, and performed several quality control steps. Transformations that failed to yield four clones were repeated (462), and those that still … See more read jarek by erin tate online free
MDM2 Regulates Dihydrofolate Reductase Activity through ...
WebConversely, the antibacterial DHFR inhibitor trimethoprim and two close structural analogs were highly selective, but weak, inhibitors of yeast complemented by the C. parvum enzyme. Future chemical refinement of the potent and selective lead compounds identified in this study may allow the design of an efficacious antifolate drug for the ... WebMay 1, 2008 · B, yeast two-hybrid screen shows that DHFR interacts with MDM2 in this system. Histogram shows the results of quantitation of the interaction compared with the … WebSep 30, 1997 · The development and wide use of synthetic antimalarial drugs in the latter half of the 20th century has been accompanied by the rapid genesis and spread of drug … read japanese translation